Composite
pJ116

Part:BBa_K4060115

Designed by: YU CHUAN LEE   Group: iGEM21_NYCU-Taipei   (2021-10-01)


pJ116 is one of our tandem promoter design

K206000 + J23116

These tandem promoters are composed of the pBad(BBa_K206000) and a constitutive promoter with the RFP gene on the downstream. We digested the vector fragment BBa_K206000 with PstI and SpeI and the insert fragment constitutive promoter with RFP by XbaI and PstI. We ligated these fragments together to form pJ102, pJ118, pJ110, pJ116, and pJ113.

Agarose electrophoresis in order to verify the tandem promoters. Lane 1, 100bp ladder. Lane 2, pJ116 with BamHI and PstI. Lane 3, pJ118 with BamHI and PstI. Lane 4, pJ113 with BamHI and PstI. Lane 5, pJ110 with BamHI and PstI. Lane 6, pJ102 with BamHI and PstI.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 145
    Illegal NheI site found at 168
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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